Hotspot

ndhF

SSC · 105156-107372 · 2217 bp

Back to Arthraxon

Pi 0.0037

MarkerSeek score 45.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 20
pi: 0.0037
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GTTTGCTGGCTTATTTGGCA	TGGTATGGTGGATAAGCCCT	45.0	50.0	57.5	57.8	2965-2969 bp	1.0	76.1
ndhF_p2	CGTTTGCTGGCTTATTTGGC	TGGTATGGTGGATAAGCCCT	50.0	50.0	58.7	57.8	2966-2970 bp	1.0	74.6
ndhF_p3	ACGTTTGCTGGCTTATTTGG	TGGTATGGTGGATAAGCCCT	45.0	50.0	57.3	57.8	2967-2971 bp	1.0	74.0
ndhF_p4	TGCTGGCTTATTTGGCAAAG	TGGTATGGTGGATAAGCCCT	45.0	50.0	57.3	57.8	2962-2966 bp	1.0	73.8
ndhF_p5	ATCCCGTGAAATTCTCGAGC	AGGTTTGGTGAATCGGAAACA	50.0	42.9	58.1	58.1	2985 bp	0.6	55.2