Hotspot

psbE-petL

LSC · 64426-65497 · 1072 bp

Back to Arthraxon

Pi 0.00765

MarkerSeek score 54.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1072
variable_sites: 19
pi: 0.00765
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GGGGTTATAGTTAAAGCAGCCA	45.0	45.5	58.0	58.1	1243-1251 bp	1.0	64.4
psbE-petL_p2	TGACAAATAACCAACCCGCA	TGGGGTTATAGTTAAAGCAGCC	45.0	45.5	58.0	58.1	1244-1252 bp	1.0	64.4
psbE-petL_p3	CAACCCGCAATGAATAGGGA	GGGGTTATAGTTAAAGCAGCCA	50.0	45.5	57.9	58.1	1232-1240 bp	1.0	64.1
psbE-petL_p4	CAACCCGCAATGAATAGGGA	TGGGGTTATAGTTAAAGCAGCC	50.0	45.5	57.9	58.1	1233-1241 bp	1.0	64.1
psbE-petL_p5	ACCAACCCGCAATGAATAGG	GGGGTTATAGTTAAAGCAGCCA	50.0	45.5	58.2	58.1	1234-1242 bp	1.0	62.6