Hotspot

petA-psbJ

LSC · 62747-63656 · 910 bp

Back to Arthraxon

Pi 0.00879

MarkerSeek score 46.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 19
pi: 0.00879
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAAAAGGTTCAATTGTACGA	ACGGTTCGTATTCTGGATTGG	33.3	47.6	53.7	58.1	979-981 bp	1.0	41.6
petA-psbJ_p2	TGAAAAGGTTCAATTGTACGAA	ACGGTTCGTATTCTGGATTGG	31.8	47.6	54.5	58.1	979-981 bp	1.0	41.6
petA-psbJ_p3	TGAAAAGGTTCAATTGTACGA	GGTTCGTATTCTGGATTGGGT	33.3	47.6	53.7	57.7	977-979 bp	1.0	41.6
petA-psbJ_p4	AGGTTCAATTGTACGAAATGA	ACGGTTCGTATTCTGGATTGG	33.3	47.6	53.5	58.1	974-976 bp	1.0	41.6
petA-psbJ_p5	TTGAAAAGGTTCAATTGTACGA	ACGGTTCGTATTCTGGATTGG	31.8	47.6	54.5	58.1	980-982 bp	1.0	41.5