Hotspot

atpI-atpH

LSC · 33742-34564 · 823 bp

Back to Arthraxon

Pi 0.00523

MarkerSeek score 67.30

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 10
pi: 0.00523
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	CAGTACCTTGACCAACTCCG	50.0	55.0	57.5	57.9	761-959 bp	1.0	80.0
atpI-atpH_p2	TAGCCGCAGCCTATATAGGT	GCAATAACAGAAGCAGCAGC	50.0	50.0	57.5	58.1	705-903 bp	1.0	80.0
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	GCAGCAATAACAGAAGCAGC	50.0	50.0	57.5	58.1	708-906 bp	1.0	80.0
atpI-atpH_p4	ATTTTTGCAACGTTAGCCGC	CAGTACCTTGACCAACTCCG	45.0	55.0	58.6	57.9	774-972 bp	1.0	79.5
atpI-atpH_p5	ATTTTTGCAACGTTAGCCGC	GCAATAACAGAAGCAGCAGC	45.0	50.0	58.6	58.1	718-916 bp	1.0	79.5