Hotspot

rpoC2-rps2

LSC · 31716-32032 · 317 bp

Back to Arthraxon

Pi 0.00694

MarkerSeek score 53.80

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 317
variable_sites: 5
pi: 0.00694
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	GAGACCCCGTTTACCCCTAT	ACCAAAATGAACTCCCGCTT	55.0	45.0	58.2	58.0	397-428 bp	1.0	83.3
rpoC2-rps2_p2	AGACCCCGTTTACCCCTATT	ACCAAAATGAACTCCCGCTT	50.0	45.0	57.7	58.0	396-427 bp	1.0	82.7
rpoC2-rps2_p3	CATCAGAGACCCCGTTTACC	ACCAAAATGAACTCCCGCTT	55.0	45.0	57.7	58.0	402-433 bp	1.0	82.4
rpoC2-rps2_p4	GAGACCCCGTTTACCCCTAT	ATGTAAGGGGCCATTTTGGG	55.0	50.0	58.2	58.1	438-469 bp	1.0	82.3
rpoC2-rps2_p5	GAGACCCCGTTTACCCCTAT	GTAAGGGGCCATTTTGGGAT	55.0	50.0	58.2	57.8	436-467 bp	1.0	81.9