Hotspot

trnC-GCA-rpoB

LSC · 20468-21602 · 1135 bp

Back to Arthraxon

Pi 0.00905

MarkerSeek score 57.70

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1135
variable_sites: 24
pi: 0.00905
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CTGGGGGTAAAGGATTTGCA	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	57.8	57.8	1290-1298 bp	1.0	78.9
trnC-GCA-rpoB_p2	CTGGGGGTAAAGGATTTGCA	TCTCAAGTTCTTCCGCCAAG	50.0	50.0	57.8	57.8	1288-1296 bp	1.0	78.9
trnC-GCA-rpoB_p3	TGGGGGTAAAGGATTTGCAG	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	57.8	57.8	1289-1297 bp	1.0	78.9
trnC-GCA-rpoB_p4	TGGGGGTAAAGGATTTGCAG	TCTCAAGTTCTTCCGCCAAG	50.0	50.0	57.8	57.8	1287-1295 bp	1.0	78.9
trnC-GCA-rpoB_p5	TGGGGGTAAAGGATTTGCAG	ACTTCTCAAGTTCTTCCGCC	50.0	50.0	57.8	57.8	1290-1298 bp	1.0	78.9