Hotspot

petN-trnC-GCA

LSC · 19453-20396 · 944 bp

Back to Arthraxon

Pi 0.00975

MarkerSeek score 58.00

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 944
variable_sites: 22
pi: 0.00975
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	CTGCAAATCCTTTACCCCCA	50.0	50.0	58.2	57.8	975-1012 bp	1.0	82.1
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	TGCAAATCCTTTACCCCCAG	50.0	50.0	58.2	57.8	974-1011 bp	1.0	82.1
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	ACTGCAAATCCTTTACCCCC	50.0	50.0	58.2	57.8	976-1013 bp	1.0	82.1
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	GCAAATCCTTTACCCCCAGT	50.0	50.0	58.2	57.8	973-1010 bp	1.0	82.1
petN-trnC-GCA_p5	AGCCCAAGCGAGACTTACTA	GTAAGGCAAGGGACTGCAAA	50.0	50.0	58.2	58.4	988-1025 bp	1.0	80.8