Hotspot

trnG-UCC-trnT-GGU

LSC · 14378-16024 · 1647 bp

Back to Arthraxon

Pi 0.00674
MarkerSeek score 56.30
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1647
variable_sites
25
pi
0.00674
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT AGCCCTTTTAACTCAGTGGT 38.1 45.0 55.5 56.7 1363-1759 bp 1.0 46.1
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT AGGCATAAGTCATCGGTTCA 38.1 45.0 55.5 56.6 1326-1722 bp 1.0 46.0
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT TGGTAAGGCATAAGTCATCGG 38.1 47.6 55.5 57.5 1331-1727 bp 1.0 45.2
trnG-UCC-trnT-GGU_p4 ACGAATCACACTTTTACCACT GGTAAGGCATAAGTCATCGGT 38.1 47.6 55.5 57.5 1330-1726 bp 1.0 45.2
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT AGCCCTTTTAACTCAGTGGT 39.1 45.0 57.3 56.7 1365-1761 bp 1.0 45.2