Hotspot

trnG-GCC-trnfM-CAU

LSC · 12991-13436 · 446 bp

Back to Arthraxon

Pi 0.0103

MarkerSeek score 58.60

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 11
pi: 0.0103
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGGAGAAGATACGGGTTCGA	TTCTTTCGTCCACCGGTTTT	50.0	45.0	57.6	58.0	579-591 bp	1.0	79.8
trnG-GCC-trnfM-CAU_p2	AGGAGAAGATACGGGTTCGA	TTTCTTTCGTCCACCGGTTT	50.0	45.0	57.6	58.0	580-592 bp	1.0	79.8
trnG-GCC-trnfM-CAU_p3	AGGAGAAGATACGGGTTCGA	TTTTCTTTCGTCCACCGGTT	50.0	45.0	57.6	58.0	581-593 bp	1.0	79.7
trnG-GCC-trnfM-CAU_p4	AGGAGAAGATACGGGTTCGA	TTTTTCTTTCGTCCACCGGT	50.0	45.0	57.6	58.0	582-594 bp	1.0	79.7
trnG-GCC-trnfM-CAU_p5	GTAGCGAGCGTAGTTCAGTG	TTCTTTCGTCCACCGGTTTT	55.0	45.0	58.5	58.0	618-630 bp	1.0	79.3