Hotspot

ycf1

SSC · 121740-126767 · 5028 bp

Back to Artemisia

Pi 0.00672

MarkerSeek score 53.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5028
variable_sites: 441
pi: 0.00672
unique_haplotype_count: 109
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCAATTGATTCAAACGTCCA	TGGTTTATCCCAAGCAACTT	35.0	40.0	53.4	55.1	259 bp	1.0	47.7
ycf1_p2	TGATTCAATTGATTCAAACGTCCA	TGGTTTATCCCAAGCAACTT	33.3	40.0	57.7	55.1	263 bp	1.0	47.6
ycf1_p3	TGATTCAATTGATTCAAACGTCCA	TTGGTTTATCCCAAGCAACT	33.3	40.0	57.7	55.1	264 bp	1.0	47.6
ycf1_p4	TGATTCAATTGATTCAAACGTCCA	TGGTTTATCCCAAGCAACTTACT	33.3	39.1	57.7	58.0	263 bp	1.0	47.6
ycf1_p5	TGATTCAATTGATTCAAACGTCCA	TTGGTTTATCCCAAGCAACTT	33.3	38.1	57.7	55.9	264 bp	1.0	47.6