Hotspot

accD

LSC · 56836-58338 · 1503 bp

Back to Artemisia

Pi 0.00502

MarkerSeek score 40.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1503
variable_sites: 79
pi: 0.00502
unique_haplotype_count: 58
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AGTTTCCCTGGATCGAGACA	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.1	58.0	2305-2514 bp	1.0	88.7
accD_p2	GAGTCCTGAACTAGCTGCTG	AAAGAAGCCATTGCGATTGC	55.0	45.0	57.7	58.0	2611-2861 bp	1.0	86.7
accD_p3	GTTTCCCTGGATCGAGACAG	AAAGAAGCCATTGCGATTGC	55.0	45.0	57.7	58.0	2304-2513 bp	1.0	86.7
accD_p4	AGTTTCCCTGGATCGAGACA	TTGCGATTGCCGGAAATACT	50.0	45.0	58.1	58.3	2295-2504 bp	1.0	86.6
accD_p5	AGTTTCCCTGGATCGAGACA	AATGAGATGTTGTGCCCTCG	50.0	50.0	58.1	58.3	2362-2571 bp	0.984127	85.6