Hotspot

ndhG-ndhI

SSC · 116704-117052 · 349 bp

Back to Artemisia

Pi 0.0101

MarkerSeek score 61.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 349
variable_sites: 49
pi: 0.0101
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TACTACTCCCAGACCCCCTA	TCAAATTGCTTTGGGTCGGT	55.0	45.0	57.7	58.3	449-554 bp	1.0	87.1
ndhG-ndhI_p2	ATACTACTCCCAGACCCCCT	TCAAATTGCTTTGGGTCGGT	55.0	45.0	58.4	58.3	450-555 bp	1.0	86.7
ndhG-ndhI_p3	AATACTACTCCCAGACCCCC	TCAAATTGCTTTGGGTCGGT	55.0	45.0	57.5	58.3	451-556 bp	1.0	85.7
ndhG-ndhI_p4	TACTACTCCCAGACCCCCTA	CAAATTGCTTTGGGTCGGTT	55.0	45.0	57.7	57.5	448-553 bp	1.0	85.3
ndhG-ndhI_p5	ATACTACTCCCAGACCCCCT	CAAATTGCTTTGGGTCGGTT	55.0	45.0	58.4	57.5	449-554 bp	1.0	84.9