Hotspot

rpl22-rps19

LSC · 82672-82763 · 92 bp

Back to Artemisia

Pi 0.0218

MarkerSeek score 62.40

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 92
variable_sites: 17
pi: 0.0218
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GAACGCCCACGAATTTGATC	GCGATAATAGGTCTCGTCGT	50.0	50.0	57.8	57.0	199-387 bp	1.0	82.0
rpl22-rps19_p2	TTACTCTTCGCGCTTTGTGA	GCGATAATAGGTCTCGTCGT	45.0	50.0	57.9	57.0	177-365 bp	0.984127	82.0
rpl22-rps19_p3	TACTCTTCGCGCTTTGTGAG	GCGATAATAGGTCTCGTCGT	50.0	50.0	58.3	57.0	176-364 bp	0.984127	80.8
rpl22-rps19_p4	GAACGCCCACGAATTTGATC	TCCGAGGACATGCAAAAAGC	50.0	50.0	57.8	59.1	217-405 bp	1.0	80.2
rpl22-rps19_p5	TTACTCTTCGCGCTTTGTGA	TCCGAGGACATGCAAAAAGC	45.0	50.0	57.9	59.1	195-383 bp	0.984127	80.1