Hotspot

ndhC-trnV-UAC

LSC · 49962-51144 · 1183 bp

Back to Artemisia

Pi 0.00824
MarkerSeek score 59.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1183
variable_sites
102
pi
0.00824
unique_haplotype_count
93
primer_available
yes
alignment_reliability
0.97
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GACTTGATATTATCAGAAATGCCCA GTTCGATCCCGTATAGCCCT 36.0 55.0 57.2 58.7 1187-1264 bp 1.0 50.7
ndhC-trnV-UAC_p2 ACTTGATATTATCAGAAATGCCCA GTTCGATCCCGTATAGCCCT 33.3 55.0 56.2 58.7 1186-1263 bp 1.0 50.7
ndhC-trnV-UAC_p3 GACTTGATATTATCAGAAATGCCCA GAAGGTCTACGGTTCGATCC 36.0 55.0 57.2 57.5 1198-1275 bp 1.0 50.6
ndhC-trnV-UAC_p4 ACTTGATATTATCAGAAATGCCCA GAAGGTCTACGGTTCGATCC 33.3 55.0 56.2 57.5 1197-1274 bp 1.0 50.6
ndhC-trnV-UAC_p5 AGACTTGATATTATCAGAAATGCCC GAAGGTCTACGGTTCGATCC 36.0 55.0 57.0 57.5 1199-1276 bp 1.0 50.6