Hotspot

trnT-UGU-trnL-UAA

LSC · 46127-46647 · 521 bp

Back to Artemisia

Pi 0.0146

MarkerSeek score 65.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 521
variable_sites: 66
pi: 0.0146
unique_haplotype_count: 71
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	GCGTCTACCTATTTCGCCAT	55.0	50.0	57.9	57.8	533-607 bp	1.0	89.7
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.8	57.8	565-639 bp	1.0	88.9
trnT-UGU-trnL-UAA_p3	ATGCGATGCTCTAACCTCTG	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.5	57.8	535-609 bp	1.0	86.6
trnT-UGU-trnL-UAA_p4	TGCGATGCTCTAACCTCTGA	GCGTCTACCTATTTCGCCAT	50.0	50.0	58.5	57.8	534-608 bp	1.0	86.6
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	AAGTCCGTAGCGTCTACCTA	55.0	50.0	57.9	57.3	542-616 bp	1.0	85.3