Hotspot

trnK-UUU-rps16

LSC · 4349-5197 · 849 bp

Back to Artemisia

Pi 0.0081
MarkerSeek score 70.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
849
variable_sites
83
pi
0.0081
unique_haplotype_count
75
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 844-981 bp 0.97619 87.4
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 843-980 bp 0.97619 87.4
trnK-UUU-rps16_p3 CCGATGGTATGGACGAATCC GGTGCTCAACCTACAGGAAC 55.0 55.0 57.9 58.2 905-1042 bp 0.968254 87.4
trnK-UUU-rps16_p4 CGATGGTATGGACGAATCCC GGTGCTCAACCTACAGGAAC 55.0 55.0 57.9 58.2 904-1041 bp 0.968254 87.4
trnK-UUU-rps16_p5 TCAGTCGTGGTCTTCCAAAC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.8 58.2 929-1066 bp 0.97619 86.6