Hotspot

trnL(CAA)-ycf2

IRa · 167613-168651 · 1039 bp

Back to Artabotrys

Pi 0.00289

MarkerSeek score 60.40

Primer available yes

Label trnL(CAA)-ycf2

Feature Metadata

label_name: trnL(CAA)-ycf2
feature_type: igs
region: IRa
length_bp: 1039
variable_sites: 3
pi: 0.00289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL(CAA)-ycf2_p1	AAATGGTAGACACGCGAGAC	GGGATGGATTTTCCCGGAAG	50.0	55.0	58.0	58.3	1184 bp	1.0	77.7
trnL(CAA)-ycf2_p2	TGAAATGGTAGACACGCGAG	GGGATGGATTTTCCCGGAAG	50.0	55.0	58.0	58.3	1186 bp	1.0	77.7
trnL(CAA)-ycf2_p3	TGGTGAAATGGTAGACACGC	GGGATGGATTTTCCCGGAAG	50.0	55.0	58.2	58.3	1189 bp	1.0	76.2
trnL(CAA)-ycf2_p4	GAAATGGTAGACACGCGAGA	GGGATGGATTTTCCCGGAAG	50.0	55.0	57.7	58.3	1185 bp	1.0	75.6
trnL(CAA)-ycf2_p5	GTTCGAGTCCTCTTCAAGGC	GGGATGGATTTTCCCGGAAG	55.0	55.0	58.3	58.3	1136 bp	1.0	75.5