Hotspot

ndhF-trnL(TAG)

IRa · 135349-137231 · 1883 bp

Back to Artabotrys

Pi 0.193

MarkerSeek score 80.10

Primer available yes

Label ndhF-trnL(TAG)

Feature Metadata

label_name: ndhF-trnL(TAG)
feature_type: igs
region: IRa
length_bp: 1883
variable_sites: 355
pi: 0.193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL(TAG)_p1	GTCGATTAAGTGCCCGAACT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	2182 bp	0.5	59.9
ndhF-trnL(TAG)_p2	GTCGATTAAGTGCCCGAACT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	2183 bp	0.5	59.9
ndhF-trnL(TAG)_p3	GTCGATTAAGTGCCCGAACT	TTTTAGAGATGCCGCCACTC	50.0	50.0	58.0	58.0	2242 bp	0.5	59.8
ndhF-trnL(TAG)_p4	GTCGATTAAGTGCCCGAACT	CTTTTAGAGATGCCGCCACT	50.0	50.0	58.0	58.0	2243 bp	0.5	59.7
ndhF-trnL(TAG)_p5	GTCGATTAAGTGCCCGAACT	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.0	58.0	2792 bp	0.5	59.6