Hotspot

ycf2-trnL(CAA)

IRb · 100610-101648 · 1039 bp

Back to Artabotrys

Pi 0.00289

MarkerSeek score 60.40

Primer available yes

Label ycf2-trnL(CAA)

Feature Metadata

label_name: ycf2-trnL(CAA)
feature_type: igs
region: IRb
length_bp: 1039
variable_sites: 3
pi: 0.00289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL(CAA)_p1	GGGATGGATTTTCCCGGAAG	AAATGGTAGACACGCGAGAC	55.0	50.0	58.3	58.0	1184 bp	1.0	77.7
ycf2-trnL(CAA)_p2	GGGATGGATTTTCCCGGAAG	TGAAATGGTAGACACGCGAG	55.0	50.0	58.3	58.0	1186 bp	1.0	77.7
ycf2-trnL(CAA)_p3	GGGATGGATTTTCCCGGAAG	TGGTGAAATGGTAGACACGC	55.0	50.0	58.3	58.2	1189 bp	1.0	76.2
ycf2-trnL(CAA)_p4	GGGATGGATTTTCCCGGAAG	GAAATGGTAGACACGCGAGA	55.0	50.0	58.3	57.7	1185 bp	1.0	75.6
ycf2-trnL(CAA)_p5	GGGATGGATTTTCCCGGAAG	GTTCGAGTCCTCTTCAAGGC	55.0	55.0	58.3	58.3	1136 bp	1.0	75.5