Hotspot

trnE(TTC)-trnT(GGT)

LSC · 33782-34604 · 823 bp

Back to Artabotrys

Pi 0.0101

MarkerSeek score 63.10

Primer available yes

Label trnE(TTC)-trnT(GGT)

Feature Metadata

label_name: trnE(TTC)-trnT(GGT)
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 8
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE(TTC)-trnT(GGT)_p1	AACTTCCCCTCGTAGTACCC	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	951-971 bp	1.0	81.8
trnE(TTC)-trnT(GGT)_p2	ACGATCAACTTCCCCTCGTA	GAACCGATGACTTACGCCTT	50.0	50.0	58.2	58.0	957-977 bp	1.0	81.7
trnE(TTC)-trnT(GGT)_p3	AACTTCCCCTCGTAGTACCC	CCATGGCGTTACTCTACCAC	55.0	55.0	58.1	58.1	930-950 bp	1.0	81.4
trnE(TTC)-trnT(GGT)_p4	ACGATCAACTTCCCCTCGTA	CCATGGCGTTACTCTACCAC	50.0	55.0	58.2	58.1	936-956 bp	1.0	81.3
trnE(TTC)-trnT(GGT)_p5	AACTTCCCCTCGTAGTACCC	TGGCGTTACTCTACCACTGA	55.0	50.0	58.1	58.1	927-947 bp	1.0	81.2