Hotspot

rpoB-trnC(GCA)

LSC · 28171-29535 · 1365 bp

Back to Artabotrys

Pi 0.0044

MarkerSeek score 60.20

Primer available yes

Label rpoB-trnC(GCA)

Feature Metadata

label_name: rpoB-trnC(GCA)
feature_type: igs
region: LSC
length_bp: 1365
variable_sites: 6
pi: 0.0044
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC(GCA)_p1	CTTCCGTCAAGCCTTGATCA	TGAACTGGGGAGAAAGGGAT	50.0	50.0	57.9	57.9	1521-1522 bp	1.0	79.0
rpoB-trnC(GCA)_p2	AGTTCTTCCGTCAAGCCTTG	TGAACTGGGGAGAAAGGGAT	50.0	50.0	58.1	57.9	1525-1526 bp	1.0	78.9
rpoB-trnC(GCA)_p3	GTTCTTCCGTCAAGCCTTGA	TGAACTGGGGAGAAAGGGAT	50.0	50.0	58.1	57.9	1524-1525 bp	1.0	78.9
rpoB-trnC(GCA)_p4	TGAAGTTCTTCCGTCAAGCC	TGAACTGGGGAGAAAGGGAT	50.0	50.0	58.1	57.9	1528-1529 bp	1.0	78.9
rpoB-trnC(GCA)_p5	GAAGTTCTTCCGTCAAGCCT	TGAACTGGGGAGAAAGGGAT	50.0	50.0	57.8	57.9	1527-1528 bp	1.0	78.5