Hotspot

trnS(GCT)-trnR(TCT)

LSC · 9108-10987 · 1880 bp

Back to Artabotrys

Pi 0.00326

MarkerSeek score 61.80

Primer available yes

Label trnS(GCT)-trnR(TCT)

Feature Metadata

label_name: trnS(GCT)-trnR(TCT)
feature_type: igs
region: LSC
length_bp: 1880
variable_sites: 6
pi: 0.00326
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(GCT)-trnR(TCT)_p1	ATTAGCAATCCGCCGCTTTA	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.0	56.9	1922-1956 bp	1.0	65.1
trnS(GCT)-trnR(TCT)_p2	ATTAGCAATCCGCCGCTTTA	AGGTTTAGAAGACCTCTGTCCT	45.0	45.5	58.0	57.9	1928-1962 bp	1.0	64.4
trnS(GCT)-trnR(TCT)_p3	CGGAGAGAGAGGGATTCGAA	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.3	56.9	1970-2004 bp	1.0	62.8
trnS(GCT)-trnR(TCT)_p4	CGGAGAGAGAGGGATTCGAA	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.3	57.9	1976-2010 bp	1.0	62.1
trnS(GCT)-trnR(TCT)_p5	GCTTTAGTCCACTCAGCCAT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.6	56.9	1908-1942 bp	1.0	62.1