Hotspot

psbA

LSC · 160-1221 · 1062 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 TGGGTATTTCTGGCTTTTCT AATACGTGTGCTTGGGAGTC 40.0 50.0 54.5 57.9 1167 bp 1.0 51.3
psbA_p2 TGGGTATTTCTGGCTTTTCTT AATACGTGTGCTTGGGAGTC 38.1 50.0 55.4 57.9 1167 bp 1.0 50.2
psbA_p3 TTGGGTATTTCTGGCTTTTCT AATACGTGTGCTTGGGAGTC 38.1 50.0 55.4 57.9 1168 bp 1.0 50.2
psbA_p4 TGGGTATTTCTGGCTTTTCTTTC AATACGTGTGCTTGGGAGTC 39.1 50.0 57.3 57.9 1167 bp 1.0 49.5
psbA_p5 TGGGTATTTCTGGCTTTTCTTT AATACGTGTGCTTGGGAGTC 36.4 50.0 56.1 57.9 1167 bp 1.0 48.4