Hotspot

rps16-trnQ-UUG

LSC · 5665-6278 · 614 bp

Back to Arrhenatherum

Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
614
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 687 bp 1.0 78.3
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 698 bp 1.0 77.8
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA AGTGTCGCATAAAACCCGAA 45.0 45.0 58.1 57.8 756 bp 1.0 77.5
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 691 bp 1.0 77.2
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC AGTGTCGCATAAAACCCGAA 50.0 45.0 58.2 57.8 767 bp 1.0 77.0