Hotspot

psbA

LSC · 495-1556 · 1062 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 AGGAGCAATAACAAACCTCTTGA ATTCATGTGCTTGGGAGTCC 39.1 50.0 58.0 57.9 1205 bp 1.0 54.8
psbA_p2 GGAGCAATAACAAACCTCTTGA ATTCATGTGCTTGGGAGTCC 40.9 50.0 56.6 57.9 1204 bp 1.0 51.9
psbA_p3 AGGAGCAATAACAAACCTCTTG ATTCATGTGCTTGGGAGTCC 40.9 50.0 56.6 57.9 1205 bp 1.0 51.8
psbA_p4 AAGGAGCAATAACAAACCTCTTG ATTCATGTGCTTGGGAGTCC 39.1 50.0 57.3 57.9 1206 bp 1.0 49.2
psbA_p5 AGGAGCAATAACAAACCTCT ATTCATGTGCTTGGGAGTCC 40.0 50.0 54.2 57.9 1205 bp 1.0 48.8