Hotspot

trnS-GCU-trnG-GCC

LSC · 8596-9258 · 663 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
663
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA AATCAAACCGAAAGACCCCT 50.0 45.0 58.0 57.0 774 bp 1.0 72.2
trnS-GCU-trnG-GCC_p2 CAATCCGACGCTTTAGTCCA ATCAAACCGAAAGACCCCTT 50.0 45.0 58.0 57.0 773 bp 1.0 72.2
trnS-GCU-trnG-GCC_p3 AATCCGACGCTTTAGTCCAC AATCAAACCGAAAGACCCCT 50.0 45.0 58.0 57.0 773 bp 1.0 72.2
trnS-GCU-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC ATCAAACCGAAAGACCCCTT 50.0 45.0 58.0 57.0 772 bp 1.0 72.2
trnS-GCU-trnG-GCC_p5 ACGGAAAGAGAGGGATTCGA ATCAAACCGAAAGACCCCTT 50.0 45.0 58.2 57.0 827 bp 1.0 71.0