Hotspot

rps16-trnQ-UUG

LSC · 6547-7354 · 808 bp

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Pi 0
MarkerSeek score 59.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
808
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 887-892 bp 1.0 78.2
rps16-trnQ-UUG_p2 CCACAGCTGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 897-902 bp 1.0 76.5
rps16-trnQ-UUG_p3 GCTTTCTGCCACATCGTTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 864-869 bp 1.0 76.5
rps16-trnQ-UUG_p4 TCCACAGCTGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 898-903 bp 1.0 76.4
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GTGTCGACTAGAAATGGGGC 50.0 55.0 58.2 58.3 949-954 bp 1.0 76.0