Hotspot

trnH-GUG-psbA

LSC · 232-494 · 263 bp

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Pi 0
MarkerSeek score 33.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
263
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 ATATTATGGGCGAACGACGG GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 441 bp 1.0 79.1
trnH-GUG-psbA_p2 ATATTATGGGCGAACGACGG CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 391 bp 1.0 78.9
trnH-GUG-psbA_p3 ATATTATGGGCGAACGACGG TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 394 bp 1.0 78.8
trnH-GUG-psbA_p4 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 380 bp 1.0 78.7
trnH-GUG-psbA_p5 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 394 bp 1.0 78.7