Hotspot

ycf1

SSC · 119303-124666 · 5364 bp

Back to Arnebia

Pi 0.0163

MarkerSeek score 51.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5364
variable_sites: 184
pi: 0.0163
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCCAGGTTTCAAGATCGGC	GAAACCTTGGCGCGTATCTA	50.0	50.0	58.0	58.1	1551-1638 bp	1.0	87.0
ycf1_p2	ATCCAGGTTTCAAGATCGGC	AGTTTGGGGAATGGAAGCTG	50.0	50.0	58.0	58.1	1473-1560 bp	0.8	78.2
ycf1_p3	ATCCAGGTTTCAAGATCGGC	CAGTTTGGGGAATGGAAGCT	50.0	50.0	58.0	58.1	1474-1561 bp	0.8	78.2
ycf1_p4	ATCCAGGTTTCAAGATCGGC	GTTTGGGGAATGGAAGCTGA	50.0	50.0	58.0	58.1	1472-1559 bp	0.8	78.1
ycf1_p5	ATCCAGGTTTCAAGATCGGC	TTGGGGAATGGAAGCTGAAC	50.0	50.0	58.0	58.1	1470-1557 bp	0.8	78.1