Hotspot

psaJ-rpl33

LSC · 64183-64599 · 417 bp

Back to Arnebia

Pi 0.0296

MarkerSeek score 72.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 417
variable_sites: 24
pi: 0.0296
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	GGACATCTTTACCCTTGGCC	45.0	55.0	58.1	58.2	453-465 bp	1.0	84.8
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	GACACAAGCGGTACATTCCA	45.0	50.0	58.1	58.2	488-500 bp	1.0	84.6
psaJ-rpl33_p3	ATGCGTTGACATTCCCCTTT	ACACAAGCGGTACATTCCAA	45.0	45.0	58.1	57.7	487-499 bp	1.0	84.1
psaJ-rpl33_p4	TCCAGATGCGTTGACATTCC	GACACAAGCGGTACATTCCA	50.0	50.0	58.3	58.2	493-505 bp	1.0	83.0
psaJ-rpl33_p5	TGCGTTGACATTCCCCTTTT	GACACAAGCGGTACATTCCA	45.0	50.0	58.3	58.2	487-499 bp	1.0	82.8