Hotspot

ycf4-cemA

LSC · 57345-58307 · 963 bp

Back to Arnebia

Pi 0.0247

MarkerSeek score 63.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 963
variable_sites: 45
pi: 0.0247
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACCCGTACTG	GGATTTCCCGAGATTCCACC	55.0	55.0	58.0	58.0	630-1198 bp	1.0	87.1
ycf4-cemA_p2	ATTCCCTTGACCCGTACTGA	GGATTTCCCGAGATTCCACC	50.0	55.0	58.1	58.0	629-1197 bp	1.0	86.9
ycf4-cemA_p3	TTCCCTTGACCCGTACTGAT	GGATTTCCCGAGATTCCACC	50.0	55.0	58.1	58.0	628-1196 bp	1.0	86.9
ycf4-cemA_p4	CGGAATTGCGGGTCTCTTTA	GGATTTCCCGAGATTCCACC	50.0	55.0	58.0	58.0	876-1444 bp	1.0	86.8
ycf4-cemA_p5	TCTTTCTACGGAATTGCGGG	GGATTTCCCGAGATTCCACC	50.0	55.0	58.0	58.0	884-1452 bp	1.0	86.8