Hotspot

accD-psaI

LSC · 55878-56265 · 388 bp

Back to Arnebia

Pi 0.124

MarkerSeek score 69.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 388
variable_sites: 115
pi: 0.124
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGGCTACCAAATGGAGTCCT	GACCCACTAAAGGCACCAAA	50.0	50.0	58.0	58.0	1081-2718 bp	1.0	88.9
accD-psaI_p2	GGCTACCAAATGGAGTCCTG	GACCCACTAAAGGCACCAAA	55.0	50.0	58.0	58.0	1080-2717 bp	1.0	88.7
accD-psaI_p3	AGGCTACCAAATGGAGTCCT	TAGACCCACTAAAGGCACCA	50.0	50.0	58.0	58.0	1083-2720 bp	1.0	88.7
accD-psaI_p4	AGGCTACCAAATGGAGTCCT	CCCGCAATTCCGTAGAAAGA	50.0	50.0	58.0	58.0	1777-2493 bp	0.6	70.6
accD-psaI_p5	AGGCTACCAAATGGAGTCCT	TAAAGAGACCCGCAATTCCG	50.0	50.0	58.0	58.0	1785-2501 bp	0.6	70.6