Hotspot

trnS-GGA-rps4

LSC · 44463-44758 · 296 bp

Back to Arnebia

Pi 0.0198

MarkerSeek score 69.80

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 296
variable_sites: 12
pi: 0.0198
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	TTTGACCCTTCACCCATTCG	50.0	50.0	58.1	58.1	448-461 bp	1.0	82.7
trnS-GGA-rps4_p2	GGCTTTTGTTTACCGAGGGT	TTTGACCCTTCACCCATTCG	50.0	50.0	58.4	58.1	447-460 bp	1.0	80.4
trnS-GGA-rps4_p3	AGGCTTTTGTTTACCGAGGG	GACCCTTCACCCATTCGAAT	50.0	50.0	58.1	57.6	445-458 bp	1.0	80.1
trnS-GGA-rps4_p4	ATGTAGGAGAGATGGCCGAG	TTTGACCCTTCACCCATTCG	55.0	50.0	58.4	58.1	500-513 bp	1.0	80.0
trnS-GGA-rps4_p5	TTCACTCGGTTTTTGGGTCA	TTTGACCCTTCACCCATTCG	45.0	50.0	57.6	58.1	528-541 bp	1.0	79.5