Hotspot

rpoB-trnC-GCA

LSC · 26308-27537 · 1230 bp

Back to Arnebia

Pi 0.02

MarkerSeek score 72.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1230
variable_sites: 52
pi: 0.02
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCATTTGCATTCCCGAGCAT	CCGGATTTGAACTGGGGAAA	45.0	50.0	58.2	58.1	1288-1312 bp	1.0	82.8
rpoB-trnC-GCA_p2	TCATTTGCATTCCCGAGCAT	AAAAGGATTTGCAGTCCCCC	45.0	50.0	58.2	58.4	1270-1294 bp	1.0	80.7
rpoB-trnC-GCA_p3	TTCCCTCATTTGCATTCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.5	58.1	1293-1317 bp	1.0	80.4
rpoB-trnC-GCA_p4	TTCCCTCATTTGCATTCCCG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.5	58.4	1275-1299 bp	1.0	78.3
rpoB-trnC-GCA_p5	CTCATTTGCATTCCCGAGCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.9	58.1	1289-1313 bp	1.0	77.4