Hotspot

atpF-atpH

LSC · 12277-12663 · 387 bp

Back to Arnebia

Pi 0.0331

MarkerSeek score 71.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 387
variable_sites: 28
pi: 0.0331
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCCCAAAGAAACGAAAGA	GGCTGGTTGTAGCATTAGCA	45.0	50.0	57.9	58.2	439-473 bp	1.0	86.3
atpF-atpH_p2	TGGCCCAAAGAAACGAAAGA	TGGGCTGGTTGTAGCATTAG	45.0	50.0	57.9	57.6	441-475 bp	1.0	85.0
atpF-atpH_p3	TGGCCCAAAGAAACGAAAGA	GCTGGTTGTAGCATTAGCAC	45.0	50.0	57.9	57.2	438-472 bp	1.0	81.7
atpF-atpH_p4	TGGCCCAAAGAAACGAAAGA	TTTATGGGCTGGTTGTAGCA	45.0	45.0	57.9	57.1	445-479 bp	1.0	81.2
atpF-atpH_p5	TGGCCCAAAGAAACGAAAGA	GGGCTGGTTGTAGCATTAGC	45.0	55.0	57.9	59.0	440-474 bp	1.0	80.5