Hotspot

atpA-atpF

LSC · 10960-11024 · 65 bp

Back to Arnebia

Pi 0.0385

MarkerSeek score 70.60

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 5
pi: 0.0385
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ATGCCATCGCCTACTTGAAG	TTGGGGGCGATGAAAGAAAT	50.0	45.0	58.0	57.8	209-212 bp	1.0	80.5
atpA-atpF_p2	CAATGCCATCGCCTACTTGA	TTGGGGGCGATGAAAGAAAT	50.0	45.0	58.3	57.8	211-214 bp	1.0	78.1
atpA-atpF_p3	AATGCCATCGCCTACTTGAA	TTGGGGGCGATGAAAGAAAT	45.0	45.0	57.6	57.8	210-213 bp	1.0	77.5
atpA-atpF_p4	ATGCCATCGCCTACTTGAAG	GTTGGGGGCGATGAAAGAAA	50.0	50.0	58.0	58.7	210-213 bp	1.0	76.3
atpA-atpF_p5	GCCATCGCCTACTTGAAGTA	TTGGGGGCGATGAAAGAAAT	50.0	45.0	57.4	57.8	207-210 bp	1.0	76.1