Hotspot

trnG-UCC-trnR-UCU

LSC · 9075-9250 · 176 bp

Back to Arnebia

Pi 0.0375

MarkerSeek score 80.90

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 176
variable_sites: 15
pi: 0.0375
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	TGGAAACAAATTGCGTCCAA	50.0	40.0	58.0	56.7	303-309 bp	1.0	77.0
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	TGGAAACAAATTGCGTCCAA	55.0	40.0	58.1	56.7	306-312 bp	1.0	76.7
trnG-UCC-trnR-UCU_p3	CCCTAGCCTTCCAAGCTAAC	TGGAAACAAATTGCGTCCAA	55.0	40.0	57.7	56.7	307-313 bp	1.0	74.5
trnG-UCC-trnR-UCU_p4	CTAACGATGCGGGTTCGATT	TGGAAACAAATTGCGTCCAA	50.0	40.0	58.4	56.7	292-298 bp	1.0	74.1
trnG-UCC-trnR-UCU_p5	TAACGATGCGGGTTCGATTC	TGGAAACAAATTGCGTCCAA	50.0	40.0	58.4	56.7	291-297 bp	1.0	74.1