Hotspot

rps15-ycf1

SSC · 130389-134808 · 4420 bp

Back to Aristotelia

Pi 0.0467

MarkerSeek score 69.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 4420
variable_sites: 195
pi: 0.0467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AATCTTTCTCCCTCCCCCTC	GCACACATGCGATGTCTTAC	55.0	50.0	58.1	57.6	251 bp	0.5	55.7
rps15-ycf1_p2	AAATCTTTCTCCCTCCCCCT	GCACACATGCGATGTCTTAC	50.0	50.0	57.6	57.6	252 bp	0.5	53.4
rps15-ycf1_p3	AATCTTTCTCCCTCCCCCTC	GTCGTTTCAGTGTGGTAAGGA	55.0	47.6	58.1	57.9	355 bp	0.5	50.4
rps15-ycf1_p4	AATCTTTCTCCCTCCCCCTC	AGTCGTTTCAGTGTGGTAAGG	55.0	47.6	58.1	57.9	356 bp	0.5	50.3
rps15-ycf1_p5	AATCTTTCTCCCTCCCCCTC	TTCAGTGTGGTAAGGATGCA	55.0	45.0	58.1	56.8	350 bp	0.5	49.3