Hotspot

rpl32-trnL-UAG

SSC · 119796-120965 · 1170 bp

Back to Aristotelia

Pi 0.195

MarkerSeek score 76.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1170
variable_sites: 160
pi: 0.195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGAGTATTGGGCAGCGTTAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1305 bp	0.5	59.7
rpl32-trnL-UAG_p2	GGAGTATTGGGCAGCGTTAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1306 bp	0.5	59.7
rpl32-trnL-UAG_p3	GGAGTATTGGGCAGCGTTAA	TCATGCCCTTTTTGGACGAA	50.0	45.0	58.0	58.0	1611 bp	0.5	59.6
rpl32-trnL-UAG_p4	GGAGTATTGGGCAGCGTTAA	TGCCCTTTTTGGACGAATCA	50.0	45.0	58.0	58.0	1608 bp	0.5	59.6
rpl32-trnL-UAG_p5	GGAGTATTGGGCAGCGTTAA	ACCAACGGAAAATGCCTCAT	50.0	45.0	58.0	58.1	2024 bp	0.5	59.1