Hotspot

ndhF-rpl32

SSC · 118428-119621 · 1194 bp

Back to Aristotelia

Pi 0.167

MarkerSeek score 71.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1194
variable_sites: 95
pi: 0.167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.48
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGTAGAAGTCCCGCTGCTAT	TTAACGCTGCCCAATACTCC	50.0	50.0	57.9	58.0	796-1365 bp	1.0	90.3
ndhF-rpl32_p2	AAGTAGAAGTCCCGCTGCTA	TTAACGCTGCCCAATACTCC	50.0	50.0	58.2	58.0	797-1366 bp	1.0	89.7
ndhF-rpl32_p3	AGTAGAAGTCCCGCTGCTAT	TTCAACGACAACGACCCATT	50.0	45.0	57.9	58.0	1812 bp	0.5	59.1
ndhF-rpl32_p4	AGTAGAAGTCCCGCTGCTAT	TTTCAACGACAACGACCCAT	50.0	45.0	57.9	58.0	1813 bp	0.5	59.1
ndhF-rpl32_p5	AGTAGAAGTCCCGCTGCTAT	TCGAGTCAGGACGATTCAGA	50.0	50.0	57.9	57.9	1471 bp	0.5	58.5