Hotspot

rps18-rpl20

LSC · 73655-73956 · 302 bp

Back to Aristotelia

Pi 0.0976

MarkerSeek score 81.20

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 302
variable_sites: 28
pi: 0.0976
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	GAGTCAACCGCCAGAACTAC	TGATTTCCAATGAGATCATAAAA	55.0	26.1	58.3	52.1	363-373 bp	1.0	49.3
rps18-rpl20_p2	GAGTCAACCGCCAGAACTAC	TGTCTTTATATGATTTCCAATGAGA	55.0	28.0	58.3	54.3	373-383 bp	1.0	49.1
rps18-rpl20_p3	GTCAACCGCCAGAACTACTG	TGTCTTTATATGATTTCCAATGAGA	55.0	28.0	58.6	54.3	371-381 bp	1.0	49.1
rps18-rpl20_p4	GAGTCAACCGCCAGAACTAC	TTGTCTTTATATGATTTCCAATGAGA	55.0	26.9	58.3	55.0	374-384 bp	1.0	49.1
rps18-rpl20_p5	TGAAAGAAGCGAGTCAACCG	TGTCTTTATATGATTTCCAATGAGA	50.0	28.0	58.5	54.3	383-393 bp	1.0	48.9