Hotspot

psbE-petL

LSC · 69733-71078 · 1346 bp

Back to Aristotelia

Pi 0.0761

MarkerSeek score 88.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1346
variable_sites: 94
pi: 0.0761
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 88.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.9	58.0	1491-1516 bp	1.0	88.6
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.9	58.0	1493-1518 bp	1.0	88.5
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	1414-1439 bp	1.0	88.0
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	1416-1441 bp	1.0	88.0
psbE-petL_p5	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.2	58.0	1500-1525 bp	1.0	87.6