Hotspot

rbcL-accD

LSC · 61132-61721 · 590 bp

Back to Aristotelia

Pi 0.0619

MarkerSeek score 87.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 36
pi: 0.0619
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 87.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	TAGAGCTTTCTTGCCCCTCT	55.0	50.0	58.0	58.0	759-818 bp	1.0	83.1
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	GAGCTTTCTTGCCCCTCTTT	55.0	50.0	58.0	58.1	757-816 bp	1.0	82.8
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	ACATCGAATTGAACCGCCAT	55.0	45.0	58.0	58.2	786-845 bp	1.0	81.3
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	TAGAGCTTTCTTGCCCCTCT	55.0	50.0	57.7	58.0	745-804 bp	1.0	81.2
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	CCATAGAGCTTTCTTGCCCC	55.0	55.0	58.0	58.3	762-821 bp	1.0	80.9