Hotspot

ndhC-trnV-UAC

LSC · 54779-55888 · 1110 bp

Back to Aristotelia

Pi 0.108
MarkerSeek score 69.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1110
variable_sites
46
pi
0.108
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.38
markerseek_score
69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCCAGAAAATATCAAAATCA AGAAGGTCTACGGTTCGAGT 31.8 50.0 54.3 57.8 531-1185 bp 1.0 48.3
ndhC-trnV-UAC_p2 AGAAATGCCCAGAAAATATCA AGAAGGTCTACGGTTCGAGT 33.3 50.0 53.1 57.8 536-1190 bp 1.0 48.3
ndhC-trnV-UAC_p3 AGAAATGCCCAGAAAATATCAA AGAAGGTCTACGGTTCGAGT 31.8 50.0 54.0 57.8 536-1190 bp 1.0 48.3
ndhC-trnV-UAC_p4 TGCCCAGAAAATATCAAAATCA GAAGGTCTACGGTTCGAGTC 31.8 55.0 54.3 57.5 530-1184 bp 1.0 48.3
ndhC-trnV-UAC_p5 TGCCCAGAAAATATCAAAATCAT AGAAGGTCTACGGTTCGAGT 30.4 50.0 54.9 57.8 531-1185 bp 1.0 48.3