Hotspot

trnC-GCA-petN

LSC · 29825-30676 · 852 bp

Back to Aristotelia

Pi 0.129

MarkerSeek score 91.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 852
variable_sites: 107
pi: 0.129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 91.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TATATCGTCTTGGCGGCATG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	57.9	57.6	1032-1087 bp	1.0	86.4
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	979-1034 bp	1.0	86.4
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	997-1052 bp	1.0	84.2
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TACCATTAACGCAGACCAGG	50.0	50.0	58.1	57.3	930-985 bp	1.0	83.9
trnC-GCA-petN_p5	TATATCGTCTTGGCGGCATG	TACCATTAACGCAGACCAGG	50.0	50.0	57.9	57.3	983-1038 bp	1.0	83.8