Hotspot

rpoB-trnC-GCA

LSC · 28684-29743 · 1060 bp

Back to Aristotelia

Pi 0.0459

MarkerSeek score 82.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1060
variable_sites: 46
pi: 0.0459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	57.9	50.0	58.0	58.1	1143-1279 bp	1.0	77.8
rpoB-trnC-GCA_p2	ATTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1148-1284 bp	1.0	77.2
rpoB-trnC-GCA_p3	TCATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.2	58.1	1142-1278 bp	1.0	76.7
rpoB-trnC-GCA_p4	AAGCCCTGATCAATGAACCT	CCGGATTTGAACTGGGGAAA	45.0	50.0	56.8	58.1	1214-1350 bp	1.0	75.8
rpoB-trnC-GCA_p5	CTCATTTCCATCCCCGAGC	AAAAGGATTTGCAGTCCCCC	57.9	50.0	58.0	58.4	1125-1261 bp	1.0	75.7