Hotspot

atpF-atpH

LSC · 14711-15205 · 495 bp

Back to Aristotelia

Pi 0.0915

MarkerSeek score 85.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 43
pi: 0.0915
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGACCGAAGAAAACGA	GCATTGGCGCTTTTATTTGC	50.0	45.0	58.1	57.6	573-648 bp	1.0	84.3
atpF-atpH_p2	CCAGTGACCGAAGAAAACGA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.1	57.3	650-725 bp	1.0	81.3
atpF-atpH_p3	CCAGTGACCGAAGAAAACGA	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.1	58.7	651-726 bp	1.0	81.2
atpF-atpH_p4	CCAGTGACCGAAGAAAACGA	GAGGCGGAGGGAAAAATACG	50.0	55.0	58.1	58.7	654-729 bp	1.0	81.1
atpF-atpH_p5	CCAGTGACCGAAGAAAACGA	GGCGGAGGGAAAAATACGAG	50.0	55.0	58.1	58.7	652-727 bp	1.0	81.1