Hotspot

trnS-GCU-trnG-UCC

LSC · 9575-10582 · 1008 bp

Back to Aristotelia

Pi 0.132

MarkerSeek score 77.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1008
variable_sites: 74
pi: 0.132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.56
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	CGAAGGACCCCTTAACTATGA	50.0	47.6	58.0	56.5	910-1119 bp	1.0	70.1
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	CGAAGGACCCCTTAACTATGA	50.0	47.6	58.0	56.5	909-1118 bp	1.0	70.1
trnS-GCU-trnG-UCC_p3	GCTCGTACAACGGATTAGCA	CGAAGGACCCCTTAACTATGA	50.0	47.6	58.1	56.5	928-1137 bp	1.0	69.4
trnS-GCU-trnG-UCC_p4	TGCTCGTACAACGGATTAGC	CGAAGGACCCCTTAACTATGA	50.0	47.6	58.1	56.5	929-1138 bp	1.0	69.3
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	CGAAGGACCCCTTAACTATGA	50.0	47.6	58.2	56.5	964-1173 bp	1.0	68.6