Hotspot

rps15-trnN-GUU

SSC · 127357-133737 · 6381 bp

Back to Aristolochia

Pi 0.0322

MarkerSeek score 69.70

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: SSC
length_bp: 6381
variable_sites: 1086
pi: 0.0322
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	GTTCTGCCTCCTTCTTGCTT	AAGGTCACACGCGAAGTATC	50.0	50.0	58.1	58.0	1120-1171 bp	0.589744	71.9
rps15-trnN-GUU_p2	TACATCCAGGCGCATTTCTC	AAGGTCACACGCGAAGTATC	50.0	50.0	58.0	58.0	1287-1338 bp	0.589744	71.5
rps15-trnN-GUU_p3	AAGAAATTGCCCATCCCGTT	AAGGTCACACGCGAAGTATC	45.0	50.0	58.1	58.0	1389-1440 bp	0.589744	71.1
rps15-trnN-GUU_p4	AGAAATTGCCCATCCCGTTT	AAGGTCACACGCGAAGTATC	45.0	50.0	58.1	58.0	1388-1439 bp	0.589744	71.1
rps15-trnN-GUU_p5	TCCAAAATCTTCTTGGCCCC	AAGGTCACACGCGAAGTATC	50.0	50.0	58.1	58.0	984-1023 bp	0.564103	68.9