Hotspot

ndhF-rpl32

SSC · 116194-117089 · 896 bp

Back to Aristolochia

Pi 0.0438
MarkerSeek score 81.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
896
variable_sites
202
pi
0.0438
unique_haplotype_count
37
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGGTATGATCCATGCATATTGA GTACGTTTCTTTGGAACTGCC 36.4 47.6 54.8 57.7 954-1257 bp 1.0 52.0
ndhF-rpl32_p2 AAGGTATGATCCATGCATATTGA GTACGTTTCTTTGGAACTGCC 34.8 47.6 55.6 57.7 955-1258 bp 1.0 52.0
ndhF-rpl32_p3 AGGTATGATCCATGCATATTGAT GTACGTTTCTTTGGAACTGCC 34.8 47.6 55.4 57.7 954-1257 bp 1.0 52.0
ndhF-rpl32_p4 AGGTATGATCCATGCATATTGA TACGTTTCTTTGGAACTGCC 36.4 45.0 54.8 56.3 953-1256 bp 1.0 52.0
ndhF-rpl32_p5 AAAGGTATGATCCATGCATATTGA GTACGTTTCTTTGGAACTGCC 33.3 47.6 56.3 57.7 956-1259 bp 1.0 52.0